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PhyloChip Technology for Simultaneous Microbial Taxa Profiling
Metagenomic analysis of environmental diversity is an area of research that continues to rapidly expand in microbiology. Approaches to analyze DNA or RNA samples from soil, water, air, and biofilms...
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The Difference Between the Nanodrop and Fluorescent Dye for Quantifying DNA
Today I wanted to talk about a method that you’ve been doing forever in your science career. Something so basic, so easy, that I bet you don’t even have to think about it. I bet you can do this type of...
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Five Ways to Save Time in the Lab and Spend More Time Outdoors
The weather is finally getting warm which means that we all want to spend more time outdoors getting UV irradiated by natural sunlight instead of under the fluorescent lightbulbs of our labs. That...
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Protect your RNA by Working Smarter and Faster
Isolation of RNA, no matter what the source, is nerve wracking, but especially when samples are limited or irreplaceable. Because RNA is so labile, working quickly but carefully is the key. There are...
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High Quality RNA In, Accurate Results Out: How to QC Your RNA
Tips and tricks for isolation of RNA from cells and tissues remains a very popular subject. I can’t tell you how many discoveries in science depend on identifying that elusive low-copy mRNA with the...
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Get to the Root of Plant DNA and RNA Isolation
If you’ve figured out how to extract high quality DNA from an elephant, chances are that without too much trouble, you’d be able to do the same from a moose, a mouse or even a meerkat. However, if...
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Choosing a Bead Tube: 101
When it comes to isolating DNA and RNA from all kinds of samples, the fastest and most thorough approach is bead beating. Whether you have microbes, mouse tissues, plant seeds and leaves, or difficult...
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Filamentous Fungus Amongus!
Our team came back from the 4th Annual Argonne Soil Metagenomics Conference held in the Chicago area October 3-5 last week and a hot topic of discussion was filamentous fungi. Seems that ears were...
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Where, Oh Where, Did My DNA Go…. a post on DNA Cleanup
This week we want to discuss a technique that is very common but still causes many people to suffer from separation anxiety. What could that possibly be? It is cleaning up dirty genomic DNA. Here’s...
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Swabs Revisited
Three years ago we published a tech article in which we went into detail on using swabs with MO BIO DNA Isolation kits. Recommendations depend both on levels of PCR inhibitors and whether one wants...
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